The practice of systematically varying injection locations to prevent localized tissue damage. Repeated subcutaneous injections at the same site can cause lipodystrophy (abnormal fat distribution), scarring, or reduced absorption from damaged tissue.

Common rotation sites for subcutaneous injection include the abdomen (avoiding a 2-inch radius around the navel), outer thighs, upper arms, and upper buttocks. Each area offers slightly different absorption rates—abdominal injections typically absorb fastest. Research protocols specify injection sites for consistency; community protocols often adopt the same rotation patterns used in insulin administration.